Wet Lab Services

Wet lab services are divided into three modules: gene synthesis – protein expression – protein purification.

1. Access Methods

1.1.1 Access via Sidebar

1.1.2 Access via Conversation Page

The AI automatically determines sequence generation, recommends placing an order, and guides the user to click and enter.

2. Gene Synthesis Module

2.1 Expression Host

You may select based on sequence type: DNA sequence (codon optimization optional) or amino acid sequence (codon optimization enabled by default).

Currently, the supported expression hosts are E. coli and Pichia pastoris.

2.2 Target Sequence

You may manually upload the sequence, or upload it with one click after interacting with the AI agent.

2.3 Vector Information

You may choose:

• Cloning vectors (2 options)

• Custom vector

For E. coli expression vectors:

• Six selectable options

• Or choose “Provide Vector” and upload the required vector sequence or plasmid map

• His‑tag position and antibiotic resistance are pre‑annotated (no need to select)

For Pichia pastoris expression vectors:

• One option currently available

• User must select His‑tag position

• Antibiotic resistance is pre‑annotated (no need to select)

2.4 Restriction Enzymes & Flanking Sequences

You may specify restriction sites or sites that must be avoided.

2.5 Delivery Contents

Default deliverables:

• Standard delivery (≥2 μg plasmid + glycerol stock)

Optional:

• Stab culture

• Plasmid preparation services (100 μg, 200 μg, 500 μg)

2.6 Information Confirmation

After clicking Information Confirmation, review the information 👉 proceed to quotation, confirm the order, and wait for customer service to verify details and send the quotation.

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3 Protein Expression Module

3.1 Protein Expression Information

After placing a gene synthesis order, you may directly click Protein Expression to proceed. Sequence information, expression host, and vector will be automatically synchronized.

3.2 Expression Conditions

E. coli

• Default expression volume: 4 ml

• Induction temperature: two preset options, or specify a temperature within a reasonable range

• Inducers: two preset options, or specify a concentration within a reasonable range

Pichia pastoris

• Default expression volume: 2 ml

• Induction temperature: default 30°C, or specify another temperature

• Option to request delivery of the expression strain (default: not delivered)

Additional requirements may be added in the remarks.

• Any additional requirements may be added in the remarks section.

3.3 Information Confirmation

After clicking Information Confirmation, verify the information 👉 proceed to quotation, confirm the order, and wait for customer service to verify details and send the quotation.

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4. Protein Purification Module

4.1 Protein Purification Module

• After placing a protein expression order, you may directly click Protein Purification to proceed. Sequence information will be auto‑filled, and protein‑related parameters will be automatically calculated. Users may review the information.

  

4.2 Purification Parameters

• Confirm whether the protein contains a His‑tag

• Whether tag‑removal service is required

• Two buffer options are currently available

4.3 Quality & Delivery

• Default purity for E. coli system: >80%

• Option to request Tm value measurement

4.4 Delivery Methods

a. Delivery based on expression volume

(Protein amount not guaranteed)

• E. coli: 400 ml or 800 ml

• Pichia pastoris: 200 ml or 1000 ml

b. Delivery based on target protein mass

Feasibility depends on small‑scale expression results.

4.5 Remarks

Any additional requirements may be added in the remarks.

4.6 Information Confirmation

• After clicking Information Confirmation, review the information 👉 proceed to quotation, confirm the order, and wait for customer service to verify details and send the quotation.

  

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